max-width: 48px; Cells grown on Gibco CF1 MEFs show improved maintenance of undifferentiated hESCs compared to low-quality MEFs, as indicated by higher TRA-1-60 expression and much lower SSEA1 expression when analyzed by flow cytometry. B) CMTI-1 mouse ES cells grown on MEFs in mouse ES cell media maintain correct pluripotent morphology (clumped colonies with clear/bright borders).

Rat Embryonic Fibroblasts (REF) are used to support the growth of rat and human pluripotent stem cells [1]. REF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. .contact-card-image { display: flex; [1] Bradley, A. } REF are isolated from rat embryos and used at early passages. Gibco MEFs support healthy PSC cultures that form compact colonies with defined edges and express pluripotency markers (Figures 1 and 2). Gibco MEFs support healthy human and mouse PSC cultures. These cells are cryopreserved at P0 and delivered frozen. For a specific lot number, please search below: We are an expanding biotechnology company whose mission is the research and development of cell and cell-related products for experimental use. } REF are characterized by immunofluorescence with antibody specific to fibronectin. flex-direction: row;

.content-card-content { For Research Use Only.

Embark on the remarkable - NEW CTS lab equipment. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells. font-family: arial, helvetica, sans-serif; The typical MEF preparation time saved with Gibco MEFs.

Email us at marketing@sciencellonline.com. }
Locate your MTI-GlobalStem MEF product in the table below to find the equivalent or most closely related Gibco MEF products as well as some selected citations. Practical Approach, (ed. In addition, Gibco MEFs help ensure superior results in many applications, including reprogramming (Figure 3). } REF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

As the largest cell provider, we are available worldwide. * These MTI-GlobalStem MEF products do not have direct equivalents at this time, but we suggest the listed related products. } (1987). Human fibroblasts were reprogrammed with the CytoTune-iPSC 2.0 Sendai Reprogramming Kit and plated on Gibco CF1 MEFs or low quality CF1 MEFs. margin-left: 20px; display: block; Isolated from embryonic day 14 rat embryos. H9 ESCs were plated on Gibco CF1 MEFs or low-quality CF1 MEFs. Gibco MEFs enabled double the reprogramming efficiency compared to low quality MEFs. height: auto; font-size: 14pt;
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A) PMEF-CF-C Mouse Embryonic Fibroblasts (MEFs) seeded at a density of 5X10^4 cell/cm 2 onto 0.1% gelatin coated plates in FBS containing MEF media 24hrs after seeding. width: 400px; Gibco MEFs allow you to save more than 2 weeks (compared to the typical MEF preparation time, Figure 4), and our careful preparation and quality tests help ensure you have more confidence in your feeder-dependent cultures. Fibroblasts are large, flat, elongated (spindle-shaped) cells possessing processes extending out from the ends of … Each vial contains 1 x 10^6 cells in 1 ml volume. Read More, Our Business Hours: Monday – Friday, 8am-5pm Pacific Standard Time Shipping Policy: We offer same day shipping for all orders received before 10am Pacific Standard Time, from Monday-Thursday*, Checking "Remember Me" will let you access your shopping cart on this computer when you are logged out. REF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. This product is for research use only. These cells are cryopreserved at P0 and delivered frozen. Images show whole wells stained with alkaline phosphatase (red). REF are isolated from rat embryos and used at early passages [2]. }

113-151. flex-wrap: wrap; .contact-container { Production and analysis of chimaeras. min-width: 48px; height: 70px; .contact-card > img { It is recommended to use DMEM (Cat. You must have JavaScript enabled in your browser to utilize the functionality of this website. #0010, 0025, 0500) for culturing REF in vitro. Fibroblast, the principal active cell of connective tissue. REF from ScienCell Research Laboratories are isolated from embryonic day 14 CD® IGS rat embryos. flex-direction: column; Oxford: IRL Press. Only registered users can write reviews. Several varieties of Gibco MEFs are available to support specific applications and different individual preferences. Laboratories typically set up timed matings to embryonic day 13.5, spend approximately 1 week preparing MEFs, and may spend additional time performing quality testing. Email us at webmaster@sciencellonline.com, Have any marketing related inquiries? By contrast, hESC and mESC colonies grown on low-quality MEFs are less defined, less compact, and show indications of differentiation. Learn more ›, A34180,A34181,A34958,A34959,A34960,A34961,A34962,A34963,A34964,A34965,A34966, Gérer l'utilisation, l'information et le service de l'instrument, Consommables en plastique de culture cellulaire, Voir les liens pour Applications et techniques, Extraction et analyse de l’ADN et de l’ARN, Solutions pour les sociétés de biotechnologie, Recherche pharmaceutique et développement de médicaments, Industries pharmaceutiques et biopharmaceutiques, Spectroscopie, analyse élémentaire et isotopique, Développement du diagnostic préclinique au diagnostic compagnon, Logiciels de gestion et d’analyse de données de laboratoire, Consommables en plastique et matériel de laboratoire, Réactifs de culture cellulaire et de transfection, Colonnes de chromatographie, résines et filtres de centrifugation, Réactifs de laboratoire et produits chimiques, Fournitures, consommables en plastique et en verre pour laboratoire, Amorces/oligos, clonage et synthèse des gènes, Informatique de laboratoire à l’échelle de l’entreprise, OEM & Commercial SupplyLicences et offres commerciales, Certifications ISO du site de fabrication, Notions fondamentales en culture cellulaire Gibco, Lettres d’information électroniques et journaux, Plate-forme d’outils et d’utilitaires pour oligos, Données chiffrées utiles pour la culture cellulaire, Générateur de panels de cytométrie en flux, Outil Switch-to-Nunc pour les supports de culture, Calculateur de protocoles de transfection, KnockOut Serum Replacement – Multi-Species, KnockOut Serum Replacement or KnockOut Serum Replacement – Multi-Species, Applications de bureau et applications mobiles, Drug selection during mouse or human ESC/iPSC culture, Pre-inactivated and ready-to-use to help save time, Available in different strains, sizes and treatments to fit your needs, Highly recommended for mouse ESC/iPSC culture, For single drug selection with (G418) geneticin, Similar resistance to G418 compared to DR4 MEFs, Higher puromycin resistance compared to DR4 MEFs, For selection with the following drugs: geneticin, puromycin, hygromycin and 6­-thioguanine. DMEM with High-Glucose, L-Glutamine and Sodium Pyruvate, Dulbecco's Phosphate-Buffered Saline, 500 ml. .contact-card > h3 { #09221) supplemented with 10% fetal bovine serum (FBS, Cat. .contact-card > p {

In Teratocarcinomas and Embryonic Stem Cells: A See How ScienCell Research Laboratories is Proactively Contributing to the Fight Against SARS-CoV-2. Figure 4. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Tissue damage stimulates fibrocytes and induces the production of fibroblasts. display: flex; Gibco Mouse Embryonic Fibroblasts (MEFs) are high-quality feeder cells used to support the growth and pluripotency of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). display: flex;

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font-size: 12pt; Please, log in or register. They are manufactured and qualified by MTI-GlobalStem, a company known for their superior performing feeder cells, which are cited in hundreds of publications. Email us at techsupport@sciencellonline.com, Need help with issues concerning this website? Gibco Mouse Embryonic Fibroblasts (MEFs) are high-quality feeder cells used to support the growth and pluripotency of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Email us at sales@sciencellonline.com, Need help with scientific inquiries, technical assistance, product applications, and use? E. J. Robertson), pp. Technical Resources Rat Embryonic Fibroblasts (REF) are used to support the growth of rat and human pluripotent stem cells.

Gibco MEFs maintain pluripotency of PSC cultures. Fibroblasts make collagen fibres, glycosaminoglycans, reticular and elastic fibers, growing individuals' fibroblasts are dividing and synthesizing ground substance. Figure 2. width: 100%; They are manufactured and qualified by MTI-GlobalStem, a company known for their superior performing feeder cells, which are cited in hundreds of publications.

padding: 20px; Please visit our Distributors page to select the company nearest to your location. Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keepthe cells in liquid nitrogen until cell culture is needed for experiments. Each vial contains 1 x 10^6 cells in 1 ml volume. Not for use in diagnostic procedures. We are located at 1610 Faraday Ave, Carlsbad, CA 92008, Call us at (877) 602-8549 or (760) 602-8549.

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} hESC and mESC colonies grown on Gibco MEFs are compact and have defined edges, indicative of healthy undifferentiated ESCs. .contact-card { The absence of quality testing increases the risk of using contaminated or low-quality MEFs. High-quality Gibco MEFs yield superior results in applications such as reprogramming. Figure 3. To serve as feeder cells, REF must be treated with mitomycin C or by irradiation to prevent cell proliferation. Human ESCs (hESCs) and mouse ESCs (mESCs) were plated on Gibco CF1 MEFs or low-quality MEFs. Need help ordering from abroad? REF are negative for mycoplasma, bacteria, yeast, and fungi. font-family: arial, helvetica, sans-serif;

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