Horizontal bars show the mean. CD45+CD3–NKp46+ cells were examined for NK1.1 and CD127 expression. In contrast, the CD34+NKlin+ population cultured in only cytokines produced a noticeable fraction of wells with K562 cytotoxicity, resulting in a steeper slope (Figure 3A).
Expression and function of the interleukin 7 receptor in murine lymphocytes. LTi-like cells (NKp46–KLRG1–RORγt+CD4+, Fig. Ikuta, K. & Weissman, I.L. After ∼ 21 days progeny were analyzed by FACS. King, A.G., Kondo, M., Scherer, D.C. & Weissman, I.L. You are using a browser version with limited support for CSS. Finn, B. Schaub, in Encyclopedia of Respiratory Medicine, 2006. Representative wells were analyzed by FACS. Godfrey, D.I., Kennedy, J., Suda, T. & Zlotnik, A. J. Exp. 5B) was not reduced, but slightly enlarged, in Irf2–/– mice as compared with those in control mice (Fig. Klein F, von Muenchow L, Capoferri G, Heiler S, Alberti-Servera L, Rolink H, Engdahl C, Rolink M, Mitrovic M, Cvijetic G, Andersson J, Ceredig R, Tsapogas P, Rolink A. We use cookies to help provide and enhance our service and tailor content and ads. For the α chain (top), a Vα-gene segment rearranges to a Jα-gene segment to create a functional gene encoding the V domain. Transcriptional regulation of early B cell development. Amounts of specific mRNA were normalized to those of Gapdh mRNA. Negotiation of the T lineage fate decision by transcription-factor interplay and microenvironmental signals. Combining the cytotoxicity with the outgrowth data, we observed wells with the following patterns: (1) no (or few) cells and no cytotoxicity, (2) cells present but no cytotoxicity, and (3) cells present and cytotoxicity present. Med. Groups showing significant differences are indicated by asterisks (***P < .001, **P = .001-.01, and *P = .01-.05), by repeated-measures analysis of variance on log-transformed values with Bonferroni posttest. Indeed, when the intrathymic lymphoid restricted precursor, the ‘low CD4 precursor’ population (Wu et al., 1991a, 1991b), was transferred intrathymically, thymic CD8α+ DCs were generated and when injected intravenously, both thymic DCs and the splenic CD8α+ DC population were generated (Ardavin et al., 1993; Wu et al., 1995, 1996).

-, Gounari, F., I. Aifantis, C. Martin, H.J. As with B-cell development, the developmental stages of T cells correspond to gene rearrangement events, culminating in specificity for the TCRs.

Godfrey, D.I., Zlotnik, A. According to the unique tissue distribution of ILC populations, the liver and lung harbored high numbers of ILC1s and ILC2s, respectively (28, 35).
14, 29–47 (1996). 173, 711–720 (1991). For grouped analysis, experimental values were normalized by log transformation before 1-way (or 2-way) analysis of variance, as indicated. Statistically significant differences are marked: *P < 0.05, ns, not significant. Interleukin-7 (IL-7) promotes survival and expansion of lymphoid precursors. Statistically significant differences are marked: *P < 0.05, **P < 0.01.

Epub 2008 Aug 22. 2 using the FC500 flow cytometer (Beckman Coulter). Thus, cells committed to a particular lineage have alternative developmental choices until lineage maintenance is complete.4. Statistical analyses were performed using GraphPad Prism Software by unpaired t-test for statistical significance. CD13+ cells expressed intracellular enzymes (myeloperoxidase, lysozyme, macrosialin [CD68]) and surface receptors (CD14 and CD1a), consistent with myeloid maturation (supplemental Figure 2). This signaling causes proliferation and expression of CD4 and CD8. CD13+CD33+ cells in these cultures were tested for characteristics unique to the myeloid lineage.

Data represent the means and SD of RT–PCR trials for three independent sortings for CLPs and PD-1hi CHILPs and two independent sortings for whole CHILPs. Gene rearrangement of TCR loci. The 2 factors (stroma and HDC) were additive because the combination was significantly better than either one alone (P < .05 at 4 weeks). We next carried out flow cytometry on BM cells to examine progenitors for ILCs in Irf2–/– mice, and found that the whole CHILP population (Lin–ckit+CD127+CD25–FLT3–α4β7hi; Fig. USA 98, 14541–14546 (2001).

Such ILC1s coproduced high levels of IFN-γ and TNF and protected against infections with the intracellular parasite Toxoplasma gondii. In this second stage thymocytes express both CD4 and CD8, hence they are termed double-positive (DP) cells. Following antigen-presentation and costimulation, T cells proliferate and elicit their effector functions, including cytokine secretion. The thymus plays an integral role in both education and regulation of T-cell lineage, with development starting in the thymic cortex and ending in the medulla.

In contrast, CD34+NKlin+ cells showed a higher NKp frequency (median, 7370/106 cells; 95% CI, 3891-13 178; n = 4; P < .01) (Figure 3B). Search for other works by this author on: Innate lymphoid cells–a proposal for uniform nomenclature, Development, differentiation, and diversity of innate lymphoid cells, Innate lymphoid cells. CD13+CD56−cells can give rise to NK cells.

Radiation BM chimeras were prepared as described previously (29). ILCs are, similarly to helper T cells, classified into three major sub-populations, based on cytokine species they produce and transcription factor dependence: T-bet-dependent group 1 ILCs (ILC1s) producing IFN-γ; RORα- and GATA3-dependent ILC2s producing IL-5 and IL-13; and RORγt-dependent ILC3s producing IL-17 and IL-22. A developmental pathway involving four phenotypically and functionally distinct subsets of CD3−CD4−CD8− triple-negative adult mouse thymocytes defined by CD44 and CD25 expression.

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