Microscope objectives are held in the optical path of the microscope via a nosepiece. In addition, this microscope includes a differential interference contrast (DIC) imaging and brightfield module with visible and near-infrared (NIR) LEDs for illumination, allowing the user to place micropipettes very accurately with the aid of NIR DIC; when used in concert with epi-fluorescence, physiological changes can be monitored without having to relocate the region of interest (ROI). Published by Carl Zeiss in the late 1960s and early 1970s, these articles remain perhaps the best source of information about the technique. In order to image with this microscope, it is necessary to add scientific cameras, an epi-illumination source, and filter sets, objectives, and sample holders. IV.

A dual-objective nosepiece, compatible with M25 x 0.75-threaded objectives, lets you locate an ROI using a low-magnification objective and then image using a high-magnification objective. Click here for additional information about viewing a sample with a Cerna microscope. For alternate rail heights, see the full web presentation. This stage is compatible with our MZS500-E Piezo-Driven Insert, which adds high-resolution Z-axis adjustments. Tip if you enter the full ordernumber of an objective using the example format 440649-0000-000 or 000000-1113-106, you can obtain information about some discontinued objectives.

The support rail utilizes 95 mm rails machined to a high angular tolerance to ensure an aligned optical path and perpendicularity with the optical table.

The Chrolis sources are compatible with the epi-illuminator module via our LLG3A6 adapter, which connects and collimates any Ø3 mm LLG to a female D3T dovetail; see image to the upper right. II.

Light from the sample plane can be collected via cameras, photomultiplier tubes (PMTs), or custom setups using breadboard tops. Included are specimen thickness, depth of field, birefringence, and refractive index.

Rigid stands can also be mounted on top of the mounting platforms for independent and synchronized movement of multiple instruments, if you are interested in performing experiments simultaneously during microscopy. Filter Cube: A cube that holds filters and other optical elements at the correct orientations for microscopy. ZEISS Information 77/78: 22-26 (1971).

Its linear dovetail mounting surface allows modules to be removed when they are not needed, freeing additional workspace and opening the door to user customization. Components attach to the body by way of either a linear dovetail on the support rail, or a circular dovetail on the epi-illumination arm (on certain models). See the comprehensive list below for each included item. Online Shop Differential Interference Contrast Microscopy. A D3T female dovetail on the rear of the microscope accepts a wide range of white-light lamps.

This large surface offers access to the optical path from the front and the sides, which is ideal for positioning micromanipulators or rigid stand sample holders.

Tune Epi-Illumination Source for the Excitation and Detection of Specific Fluorophores, Up to Six Filter Sets can be Installed Simultaneously, Utilize Fluorescence Filters from Other Major Manufacturers, Microscope Accepts M25 x 0.75-Threaded Objectives (Nikon), Matching DIC Objective Prisms (Sold Separately) are Required for DIC, DIC Polarizers, Condenser Prisms, and Analyzer are Included with Microscope Kit, Rigid Stands Hold Samples Underneath and Around the Objective, Designed for Slides, Petri Dishes, Well Plates, Recording Chambers, Micromanipulators, and Custom Inserts, Translation Stages with 1" of X and Y Travel Available, Fixed Arms Incorporate Fast XY Stage, Lens Tubes, and/or Cage Systems Directly into Optical Path, CSA1000: For Our MLS203-1 Fast XY Scanning Stage, CSA1001: For Ø1" Lens Tubes and 30 mm Cage Systems, CSA1002: For Ø2" Lens Tubes and 60 mm Cage Systems, Circular Hole Designed for Recording Chambers.

The epi-fluorescence generated by the sample passes through the module to the eyepieces and camera. Zeitschrift für Wissenschaftliche Mikroskopie und Mikroskopische Technik 69: 193-221 (1969).

Trans-illumination modules, which condition light (on certain models) and direct it along the optical path, are attached to the support rail of the microscope body via a linear dovetail (see Microscope Dovetails tab or here). A review of de Sénarmont compensators and their appliation to alterations of the wavefront field in DIC microscopy.

The table below on the right gives the item number of the DIC objective prism you should order. To ensure a light-tight path from the objective, the microscope body comes with a bellows (not pictured). While both filter sets target mCherry, the -MCHC excitation filter features a broader transmission band than the excitation filter in the -MCHC filter set. The relationship between optical path gradients and intensity profiles in DIC microscopy is illustrated in Figure 2. Nomarski's DIC microscopy: A review.

Microscope objectives are available for purchase separately from Thorlabs, and we can also order other objectives outside our catalog upon request. ZEISS International. Trans-Illumination: Illumination on the opposite side of the sample as the viewing apparatus. In addition, we can work with you to configure a microscope that meets your unique needs. Several popular filter sets are listed with their target fluorophores in the table to the right. The Zeiss-Nomarski differential interference equipment for transmitted-light microscopy. Click here for additional information about viewing samples with a Cerna microscope. Nature Milestones, Milestone 8: (2009). Please see the, Visualize the Field of View on a Computer, Any C-Mount Camera is Compatible with a Cerna Microscope, White Light Sources Illuminate the Field of View Through the Objective, Light is Tuned by Filter Sets for Specific Fluorophores (See Below), Broadband LEDs Generate Intense Illumination Across the Visible Spectrum, Maximum LED Output Powers from 3.1 to 7.1 W, Combine 6 User-Changeable, High-Power LEDs into One Liquid Light Guide, Eleven Wavelength Options Cover All Known Fluorophores Between 365 nm and 780 nm (, Control LEDs Using Included Software with GUI, Typical Lifetime of LED Modules is ≥10,000 Hours. Comparison with phase contrast.

Alternatively, the CSA1001 and CSA1002 Fixed Arms are compatible with Thorlabs' extensive selection of optomechanical components, allowing custom sample holder configurations to be integrated into the microscope. Click here for additional information on trans-illumination with Cerna.

Please see the full web presentation of each to determine its functionality within the Cerna microscopy platform. Principles and applications of differential interference contrast light microscopy. Please note that certain imaging modalities will require additional optics to alter the properties of the beam; these optics may be easily incorporated in the optical path via lens tubes and cage systems. The nosepiece in this microscope kit contains M25 x 0.75 threads in two places, allowing it to hold two objectives simultaneously.

This metric microscope configuration can be tailored to your particular imaging needs through the use of our kit functionality. Multiple epi-illumination modules are available, as well as breadboard tops, which have regularly spaced tapped holes for custom designs.

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Köhler Illumination: A method of illumination that utilizes various optical elements to defocus and flatten the intensity of light across the field of view in the sample plane. The final paper in the Carl Zeiss DIC series addresses various applications of DIC microscopy in the biological and material sciences.

Please see the Microscope Dovetails tab or here for further details. 1). Nomarski differential interference-contrast microscopy. Bellows: A tube with accordion-shaped rubber sides for a flexible, light-tight extension between the microscope body and the objective. We have also indicated when it is possible to use equipment designed by other manufacturers. Please see the Kit Components tab for additional information about each component in this microscope kit. The rigid stands can translate by way of motorized stages (sold separately), while the mounting platforms contain built-in mechanics for motorized or manual translation.

This kit configuration is constructed from our modular Cerna® components. We also manufacture translation stages for these rigid stands that provide motorized horizontal translation of the sample. The first article in a four-part series outlining the basic theory and practice of differential interference contrast. Epi-illumination modules, which direct and condition light along the optical path, are attached to the epi-illumination arm of the microscope body via a circular D1N dovetail (see the Microscope Dovetails tab or here for details). Please see the Kit Components tab for additional information about each component in this microscope kit.

It is used in fluorescence, confocal, and reflected light microscopy.
II. Support

The included collar makes them lockable at a height and angle chosen by the user. On the Cerna microscope, the objective is threaded onto a nosepiece, which holds the objective at the throat depth, or the distance from the optical path to the support rail of the microscope body. Differential interference contrast microscopy (DIC; Figure 1(c)) requires plane-polarized light and additional light-shearing (Nomarski) prisms to exaggerate minute differences in specimen thickness gradients and refractive index.

This imperial microscope configuration can be tailored to your particular imaging needs through the use of our kit functionality.
Nomarski differential interference-contrast microscopy. For example, when the trans-illumination modules are installed, in vitro samples can be studied using epi-fluorescence and DIC imaging, as well as with basic widefield and brightfield illumination. Epi-Illumination: Illumination on the same side of the sample as the viewing apparatus. If you would like to work with our application specialists, engineers, and sales team to design your own microscopes, please email ImagingSales@thorlabs.com. For example, filter cubes are essential for fluorescence microscopy and reflected light microscopy. This Cerna microscope kit was designed from our line of modular components to provide several convenient features for imaging, highlighted below. The size of the throat depth, along with the working height, determine the working space available for microscopy. ¦ Thorlabs offers highly configurable solutions for mounting your sample beneath the objective of the Cerna microscope.

Please note that our translating platform comes in both imperial and metric versions. ¦

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