The effect of dietary protein on the induction of intestinal hormones is recognised.

In contrast, somatostatin cells were observed throughout the human gut, and we did not observe costaining of somatostatin with any of the other hormones tested. We thank Professor Bente Pakkenberg and Ph.D. student Anna Schou Karlsen for essential insights into stereology; and Professors Jens F. Rehfeld, Jan Fahrenkrug, and Ove Schaffalitzky de Muckadell for the generous gifts of antibodies. Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Centre (UMC) Utrecht, 3584 CT Utrecht, the Netherlands, Princess Máxima Centre for Paediatric Oncology, 3584 CT Utrecht, the Netherlands, Neurog3Chrono arranges enteroendocrine single-cell transcriptomes on real-time axis, Enteroendocrine cells show hormonal plasticity in the course of their maturation, A time-resolved map characterizes fate specification of all enteroendocrine lineages, Individual knockout of 6 identified regulators gives robust enteroendocrine phenotypes, Homeostatic regulation of the intestinal enteroendocrine lineage hierarchy is a poorly understood process.
Intestinal organoids were switched to enteroendocrine differentiation medium (. (38). The broad coexpression phenomenon was observed both in crypts and villi as demonstrated by immunohistochemistry and FACS analysis of separated cell populations. Single-cell sequencing reveals dissociation-induced gene expression in tissue subpopulations. Primers and Oligo Sequences, Related to STAR Methods, Accepted: destiny: diffusion maps for large-scale single-cell data in R. (A) Live imaging of Tac1Cre LSL-tdTomato small intestinal organoids.


2). A total number of 6906 cells were sorted and processed following the SORT-Seq method (.

These changes seemingly were not due to a generalized diphtheria toxin-induced gut mucosal injury because no obvious histological changes to the mucosal appearance were observed (Supplemental Fig.  |  The purity of the preparation was substantiated by the fact that the top nine scoring proteins, based on the sum of the single peptides recorded in the LC-MS analysis, all were derived from either gut hormone precursors or progranins. Sections were preblocked using 2% BSA (15 min room temperature) and incubated overnight at 4 C with the primary antibodies (Supplemental Table 2), washed in PBS (three times 2 min), and incubated for 1 h with biotinylated secondary antibodies (Supplemental Table 2). (B) Diffusion map visualization showing all EE progenitors in combination with all cells of the EC, ILN- and Delta-cell lineages (min. Baumgart DC, Thomas S, Przesdzing I, et al. Thus, we performed single cell sequencing on reporter-positive cells from small intestinal crypts and villi. Peptides, but not free amino acids, showed a potent GLP-1 secretagogue effect, while proteins only had a modest effect.

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