Prepare and fix the specimen smear prior to staining.

Place a small strip of blotting or filter paper over the top of the specimen, and place the slide over a boiling hot water bath on a mesh surface.

Alcohol-fixation: This is recommended when the smear has not been prepared from sodium hypochlorite … We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. 23 Procedure of Acid-Fast Stain Prepare bacterial smear on clean and grease free slide, using sterile technique.

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These acids resist staining by ordinary methods such as a Gram stain. Clipping is a handy way to collect important slides you want to go back to later. Staining procedure: Acid-fast organisms like Mycobacterium contain large amounts of lipid substances within their cell walls called mycolic acids. Z N stain is a modification of Ehrlich’s original method for differential staining of acid fast bacilli by use of aniline gentian violet followed by strong nitric acid.

It can also be used to stain a few other bacteria, such as Nocardia. The ordinary aniline dye solution do not readily penetrate the acid fast bacilli. ACID FAST STAINING PROCEDURE FOR STAINING MYCOBACTERIA
A Presentation
By
Isaac .U.M.,
Department of Microbiology & Parasitology,
Faculty of Medicine, International Medical & Technological University,
Dar-Es-Salaam, Tanzania
. Place slides on dryer with smeared surface upwards, and air dry for about 30 minutes. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising.

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If you wish to opt out, please close your SlideShare account. • Stain used consists of basic fuchsin, with phenol added. Ziehl-Neelsen (Acid Fast) Staining procedure Spread the sputum evenly over the central area of the slide using a continuous rotational movement.

Allow smear to air dry and then heat fix. Acidfast Stain: Background and Introduction. Procedure of Acid Fast Stain.

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Now customize the name of a clipboard to store your clips. I don't have enough time write it by myself. Cover the filter paper with the primary stain, carbolfuchsin. So by use of powerful staining solution that contain phenol and application of heat , the dye can be made to penetrate the bacillus.

I can recommend a site that has helped me. See our User Agreement and Privacy Policy. ACID – FAST STAINING • This is also known as Ziehl – Neelsen staining. Hi there!

Preparation of microscope slide: Clean slide with a Kimwipe and alcohol to remove any fingerprints. Leave the slide on the water bath for 3 to 5 minutes. Acid fast stainingsummary Microbiology Lab 11th SeptRavi A Patel Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising.

If you continue browsing the site, you agree to the use of cookies on this website. • This method is a modification of Ehrlich’s (1882) original method for the differential staining of tubercle bacilli and other acid-fast bacilli with aniline-gentian violet followed by strong nitric acid. You can change your ad preferences anytime. Learn more. See our Privacy Policy and User Agreement for details. Looks like you’ve clipped this slide to already. Mycobacterium and many Nocardia species are called acid-fast because during an acid-fast staining procedure they retain the primary dye carbol fuchsin despite decolorization with the powerful solvent acid-alcohol.Nearly all other genera of bacteria are nonacid-fast.The acid-fast genera have lipoidal mycolic acid in their cell walls.

Continue to apply stain if the filter paper begins to dry. Hello! I like this service ⇒ www.HelpWriting.net ⇐ from Academic Writers.

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The recommended size of the smear is about 20 mm by 10 mm. ACID FAST STAINING PROCEDURE FOR STAINING MYCOBACTERIAA Presentation ByIsaac .U.M., Department of Microbiology & Parasitology,Faculty of Medicine, Internationa… Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. If you continue browsing the site, you agree to the use of cookies on this website. Medical College, Thoothukudi, No public clipboards found for this slide, Acid fast staining procedure for staining mycobacteria, Senior Lecturer at B.K.Mody Government Pharmacy College.

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